PHENOL-CHLOROFORM.pdf
0.20MB

Introduction
A phenol-chloroform extraction is a liquid-liquid extraction. A liquid-liquid extraction is a
method that separates mixtures of molecules based on the differential solubilities of the
individual molecules in two different immiscible liquids (28). Liquid-liquid extractions are
widely used to isolate RNA, DNA, or proteins1.

Brief History
Volkin & Carter reported the first use of guanidinium chloride in the isolation of RNA in 1951
(30). In 1953, Grassmann & Defner described the efficacy of phenol at extracting proteins
from aqueous solution (16). Utilizing this find, Kirby demonstrated the use of phenol to
separate nucleic acids from proteins in 1956 (18). Cox and others renewed interest in the use
of guanidinium chloride in the isolation of RNA from ribonucleoproteins in the 1960s
(11,12,13). From then on, guanidinium extractions were the method of choice for RNA
purification, replacing phenol extraction. The use of guanidinium thiocyanate instead of
guanidinium chloride was first briefly mentioned by Ullrich et al. in 1977 (29), and later
successfully employed by Chirgwin et al. in 1979 (8). Chirgwin et al. used guanidinium
thiocyanate to isolate undegraded RNA from ribonuclease-rich tissues like pancreas. A
combination of guanidinium thiocyanate and hot phenol for RNA isolation was reported by
Feramisco et al. in 1981 (14). In 1987, Chomczynski & Sacchi combined guanidinium
thiocyanate with phenol-chloroform extraction under acidic conditions (9). Since its
inception, the Chomczynski & Sacchi method has been the method of choice to isolate RNA
from cultured cells and most animal tissues (10).

 

첨부파일 참고

Stay Healthy
Around Small Pets
꼬마 동물친구들과
안전하게 지내는 방법

https://www.cdc.gov/healthypets/publications/stay-healthy-around-small-pets.html

safety-around-small-pets-H.pdf

 

 

Pet rodents (such as mice, rats, hamsters, gerbils, and guinea pigs) and other small pets (such as hedgehogs, sugar gliders, chinchillas, and ferrets) can sometimes carry germs that can make people sick. These germs can cause illnesses ranging from serious diarrhea to birth defects.

  • Small pets can carry germs even if they look healthy and clean.
  • Germs are shed in their droppings and can easily contaminate their bodies, habitats, toys, bedding, and anything in areas where they live.
    • These germs can spread to people after they touch these animals or anything in their habitats.

But there’s good news! You can take steps to stay healthy while enjoying your pets.

 

Protect Yourself and Your Family from Germs

Pick the Right Pet for Your Family.

Rodents and other small pets are not recommended for children under 5 years old, adults over 65, or people with weakened immune systems because they are at a greater risk for serious illness from germs that pets can carry.

Wash Your Hands.

Always wash hands thoroughly with soap and water right after touching, feeding, or caring for your pets or cleaning their habitats.

  • Adults should supervise hand washing for young children.
  • If soap and water are not readily available, use hand sanitizer until you are able to wash your hands thoroughly with soap and water.

Keep it Clean.

Keep small pets and their supplies out of kitchens and other areas where food is prepared, served, stored, or consumed.

  • Clean habitats, toys, and supplies outside the house when possible.
  • If you clean supplies indoors, use a laundry sink or bathtub, and thoroughly clean and disinfect the area right after.

Play Safely.

  • Don’t kiss, snuggle, or hold small pets close to your face.
  • Don’t let them roam free in your home.
  • Don’t touch your mouth after handling small pets.
  • Don’t eat or drink around animals.

Print Version[PDF – 1 page](https://www.cdc.gov/healthypets/resources/safety-around-small-pets-H.pdf)

 

 

블라인드 채용시 유의점 알아보자.

http://www.mois.go.kr/frt/bbs/type001/commonSelectBoardArticle.do?bbsId=BBSMSTR_000000000015&nttId=59831

 

행정안전부(구)국민안전처)에서 발간한 지방기관 블라인드 채용 가이드 북에 '출제자'의 입장이 상세히 반영된,

'채용자의 관점에서 준비하는' 블라인드 채용 내용이 적혀있다.

 

 

 

지방공공기관 블라인드 채용 가이드북(게시용).pdf

 

살펴보고 자소서 작성하자!

재미있을 것 같아 우선 스크랩



남북 교류 활성화에 따른 보건의료 협력과 발전을
위한 방향 토론

영원할 것 같았던 분단의 겨울을 지나 불현 듯 봄이 오고 있습니다. 그 오랜 시간동안 남북은 대립과 극복의 대상으로 상대를 보아 오기도 하였지만 무관심의 대상이 되기도 했습니다. 이제 남과 북이 이웃으로 소통하고 거리낌 없이 나눔을 주고받는 시대가 성큼 다가온 듯 합니다. 보건의료분야는 어려움속에서도 적지 않은 소통을 해왔지만 지난 10년간은 그나마도 이루어지지 못하였습니다. 이제 다시 올 평화의 시대, 남과 북이 서로 오가고 주고받는 시대를 앞두고 보건의료분야는 공동으로 질병을 퇴치하고 환경을 개선하여 건강증진을 이룩하여야 할 것입니다. 대한예방의학회와 한국역학회는 국회 정춘숙 의원실과 함께 단절된 지난 시간동안의 남북 보건의료체계 변화를 되짚어보고 양측의 주요한 보건문제를 확인하여 앞으로 다가올 남북 평화 교류시대를 현명하게 준비하고자 합니다. 많은 관심과 참여를 기대합니다.

  • 목적 : 남북 교류 활성화에 따른 보건의료 협력과 발전을 위한 방향 토론
  • 주최 : 대한예방의학회, 한국역학회, 정춘숙(보건복지위원회 의원) 국회의원실
  • 일시 및 장소 : 6월 22일(금) 13:00-18:00, 국회도서관 B1F 소회의실


What is an epidemiological week and why do we use them?



An epidemiological week, commonly referred to as an epi week or a CDC week, is simply a standardized method of counting weeks to allow for the comparison of data year after year. Many people know these as CDC weeks because CDC uses them and has published them in the past. Nevertheless, they are used throughout the world by epidemiological teams in different countries. Unfortunately, not all countries calculate epi weeks in the same manner, and this discrepancy causes some difficulty in comparing data internationally. 


The question is, how do we define epi weeks? The heart of the matter is how we define the first epi week. Is it the week in which the first of January falls? Is it the first complete week in January? To solve this, there is a definition (in the United States and many Latin American countries) as to how we establish epi week one.

The first epi week of the year ends, by definition, on the first Saturday of January, as long as it falls at least four days into the month. Each epi week begins on a Sunday and ends on a Saturday.
(http://www.paho.org/english/sha/be_v21n3-calendar.htm)
The following figure provides the epi week calendar for 2006, with epi week one starting (by coincidence) on the first of January.


So why do we bother to determine and use epi weeks? Many times, particularly for mosquito surveillance programs or epidemiological studies, daily increments are too frequent and too varied to be able to be managed and analyzed, or there are many factors that make it impossible to compare daily results. On the other hand, the monthly time interval is too great, and data interpretation is needed at more frequent intervals. Therefore we seek an intermediary period of time to analyze the data, and this brings us to the week. By defining the epi week, we are able to maintain harmony and ensure that all parties count weeks the same way, understanding that epi week n refers to the same time period.



Jorge R. Arias, Ph.D.

Disease Carrying Insects Program

Fairfax County, Virginia

(source: https://www.cmmcp.org/sites/cmmcp/files/uploads/spring_skeeter_06.pdf)

Using Conjunctive Adverbs

 

Use conjunctive adverbs (or sentence adverbs) to:

  • indicate a connection between two independent clauses in one sentence
  • link the ideas in two or more sentences
  • show relationships between ideas within an independent clause.

Examples of Conjunctive Adverbs:

  • also
  • however
  • otherwise
  • consequently
  • indeed
  • similarly
  • finally
  • likewise
  • then
  • furthermore
  • moreover
  • therefore
  • hence
  • nevertheless
  • thus
  • nonetheless

How to punctuate conjunctive adverbs

When a conjunctive adverb connects two independent clauses in one sentence, it is preceded by a semicolon and followed by a comma.

Tuition increases, say officials, are driven by the universities' costs; consequently, tuition income typically covers less than 50% of college budgets.


If a conjunctive adverb is used in any other position in a sentence, it is set off by commas.

Nonetheless, some colleges are making efforts to trim budgets and pass along the savings.

Secretary Bennett, however, maintains that more federal aid would only encourage universities to count on the government to meet any increases they might impose.


— All examples taken from "Facing Up to Sticker Shock," Time (April 20, 1987), 70.


https://writing.wisc.edu/Handbook/ConjAdv.html


As a "part of speech," transition words are used to link words, phrases or sentences. They help the reader to progress from one idea (expressed by the author) to the next idea. Thus, they help to build up coherent relationships within the text.

Transitional Words

This structured list of commonly used English transition words — approximately 200, can be considered as quasi-complete. It can be used (by students and teachers alike) to find the right expression. English transition words are essential, since they not only connect ideas, but also can introduce a certain shift, contrast or opposition, emphasis or agreement, purpose, result or conclusion, etc. in the line of argument. 
The transition words and phrases have been assigned only once to somewhat artificial categories, although some words belong to more than one category.

There is some overlapping with prepositions and postpositions, but for the purpose of usage and completeness of this concise guide, I did not differentiate.

Agreement / Addition / Similarity

The transition words like also, in addition, and, likewiseadd informationreinforce ideas, and express agreement with preceding material.

 

in the first place

not only ... but also

as a matter of fact

in like manner

in addition

coupled with

in the same fashion / way

first, second, third

in the light of

not to mention

to say nothing of

equally important

by the same token

again

to

and

also

then

equally

identically

uniquely

like

as

too

moreover

as well as

together with

of course

likewise

comparatively

correspondingly

similarly

furthermore

additionally

 

 

Opposition / Limitation / Contradiction

Transition phrases like butrather and or, express that there is evidence to the contrary or point out alternatives, and thus introduce a change in the line of reasoning (contrast).

 

although this may be true

in contrast

different from

of course ..., but

on the other hand

on the contrary

at the same time

in spite of

even so / though

be that as it may

then again

above all

in reality

after all

but

(and) still

unlike

or

(and) yet

while

albeit

besides

as much as

even though

although

instead

whereas

despite

conversely

otherwise

however

rather

nevertheless

nonetheless

regardless

notwithstanding

 

 

Cause / Condition / Purpose

These transitional phrases present specific conditions or intentions.

 

in the event that

granted (that)

as / so long as

on (the) condition (that)

for the purpose of

with this intention

with this in mind

in the hope that

to the end that

for fear that

in order to

seeing/being that

in view of

If

... then

unless

 

when

whenever

while

 

because of

as

since

while

lest

in case

provided that

given that

only / even if

so that

so as to

owing to

inasmuch as

due to

 

Examples / Support / Emphasis

These transitional devices (like especially) are used to introduce examples as support, to indicate importance or as an illustration so that an idea is cued to the reader.

 

in other words

to put it differently

for one thing

as an illustration

in this case

for this reason

to put it another way

that is to say

with attention to

by all means

 

 

 

important to realize

another key point

first thing to remember

most compelling evidence

must be remembered

point often overlooked

to point out

on the positive side

on the negative side

with this in mind

notably

including

like

to be sure

namely

chiefly

truly

indeed

certainly

surely

markedly

such as

 

especially

explicitly

specifically

expressly

surprisingly

frequently

significantly

particularly

in fact

in general

in particular

in detail

for example

for instance

to demonstrate

to emphasize

to repeat

to clarify

to explain

to enumerate

 

 

Effect / Consequence / Result

Some of these transition words (thus, then, accordingly, consequently, therefore, henceforth) are time words that are used to show that after a particular time there was a consequence or an effect.

Note that for and because are placed before the cause/reason. The other devices are placed before the consequences or effects.

 

as a result

under those circumstances

in that case

for this reason

in effect

for

thus

because of

then

hence

consequently

therefore

thereupon

forthwith

accordingly

henceforth

 

 

Conclusion / Summary / Restatement

These transition words and phrases concludesummarize and / or restate ideas, or indicate a final general statement. Also some words (like therefore) from the Effect / Consequence category can be used to summarize.

 

as can be seen

generally speaking

in the final analysis

all things considered

as shown above

in the long run

given these points

as has been noted

in a word

for the most part

after all

in fact

in summary

in conclusion

in short

in brief

in essence

to summarize

on balance

altogether

overall

ordinarily

usually


by and large

to sum up

on the whole

in any event

in either case

all in all

 

Obviously

Ultimately

Definitely

 

Time / Chronology / Sequence

These transitional words (like finally) have the function of limiting, restricting, and defining time. They can be used either alone or as part of adverbial expressions.

 

at the present time

from time to time

sooner or later

at the same time

up to the present time

to begin with

in due time

as soon as

as long as

in the meantime

in a moment

without delay

in the first place

all of a sudden

at this instant

first, second

 

immediately

quickly

finally

after

later

last

until

till

since

then

before

hence

since

when

once

about

next

now

 

 

formerly

suddenly

shortly



henceforth

whenever

eventually

meanwhile

further

during

in time

prior to

forthwith

straightaway

 

by the time

whenever

 

until now

now that

 

instantly

presently

occasionally

 

 

Many transition words in the time category (consequently; first, second, third; further; hence; henceforth; since; then, when; and whenever) have other uses.

Except for the numbers (first, second, third) and further they add a meaning of timein expressing conditions, qualifications, or reasons. The numbers are also used to add information or list examplesFurther is also used to indicate added space as well as added time.

 

Space / Location / Place

These transition words are often used as part of adverbial expressions and have the function to restrict, limit or qualify space. Quite a few of these are also found in the Time category and can be used to describe spatial order or spatial reference.

 

in the middle

to the left/right

in front of

on this side

in the distance

here and there

in the foreground

in the background

in the center of

 

adjacent to

opposite to 

here

there

next

where

from

over

near

above

below

down

up

under



further

beyond

nearby

wherever

around

between

before

alongside

amid

among

beneath

beside

behind

ac


http://www.smart-words.org/linking-words/conjunctions.html



Words which connect words, phrases, clauses or sentences are called conjunctions (see "to conjoin" = join, unite). The most common ones are 'and', 'or' and 'but'. These words all have different nuances and connotations but they all help to build up meaningful relationships within a sentence.

Conjunctions

Cohesive Devices

A variety of useful English Conjunctions exists, which complete this list of the most used Cohesive Devices. Together, they can help to express a cohesive view and easy understandable and readable texts.

There are three basic types of conjunctions:

Definition

coordinating conjunctions
used to connect two independent clauses
subordinating conjunctions
used to establish the relationship between the dependent clause and the rest of the sentence
correlative conjunctions
used to join various sentence elements which are grammatically equal

Coordinating Conjunctions

Comes usually in the middle of a sentence, and a comma is used before the conjunction (unless both clauses are very short). They join individual words, phrases, and independent clauses. 
Whereas coordinating conjunctions join parts of a sentence, the purpose of transitional words and phrases usually is to join two 'sentences'.

Examples: 
We can draw lessons from the past, but we cannot live in it. [Lyndon B. Johnson]
The purpose of most computer languages is to lengthen your resume by a word anda comma. [Larry Wall]

Andbutfornororso, and yet — are the seven coordinating conjunctions. To remember them, the acronym FANBOYS can be used.

  1.  F = for

  2.  A = and

  3.  N = nor

  4.  B = but

  5.  O = or

  6.  Y = yet

  7.  S = so

Subordinating Conjunctions

Also called subordinators, introduce a dependent clause. These adverbs that act like conjunctions are placed at the front of the clause - and a comma is needed at the end of the adverbial phrase when it precedes the main clause. 

Examples: 
If the only tool you have is a hammer, you tend to see every problem as a nail. [Abraham Maslow]
Some people make headlines while others make history. [Philip Elmer-DeWitt]

 

ConjunctionsConcession

  • though
  • although
  • even though
  • while

ConjunctionsCondition

  • if
  • only if
  • unless
  • until
  • provided that
  • assuming that
  • even if
  • in case (that)
  • lest

ConjunctionsComparison

  • than
  • rather than
  • whether
  • as much as
  • whereas

Conjunctions Time

  • after
  • as long as
  • as soon as
  • before
  • by the time
  • now that
  • once
  • since
  • till
  • until
  • when
  • whenever
  • while

 

Conjunctions Reason

  • because
  • since
  • so that
  • in order (that)
  • why

Relative Adjective

  • that
  • what
  • whatever
  • which
  • whichever
  •  

Relative Pronoun

  • who
  • whoever
  • whom
  • whomever
  • whose
  •  
  •  

ConjunctionsManner

  • how
  • as though
  • as if

Conjunctions Place

  • where
  • wherever

 

Correlative Conjunctions

They are always used in pairs and denote equality; and show the relationship between ideas expressed in different parts of a sentence - and thus make the joining tighter and more emphatic. When joining singular and plural subjects, the subject closest to the verb determines whether the verb is singular or plural. 

 

  • as . . . as
  • just as . . . so
  • both . . . and
  • hardly . . . when
  • scarcely . . . when
  • either . . . or
  • neither . . . nor
  •  
  • if . . . then
  • not . . . but
  • what with . . . and
  • whether . . . or
  • not only . . . but also
  • no sooner . . . than
  • rather . . . than

 

Conjunctive Adverbs

They are often used as a linking device between ideas. They show logical relationships expressed in clauses, sentences or paragraphs.
Conjunctive adverbs are very emphatic, so they should be used sparingly.

 

Similar to And

  •  
  • also
  • besides
  • furthermore
  • likewise
  • moreover

Similar to But

  •  
  • however
  • nevertheless
  • nonetheless
  • still
  •  
  • conversely
  • instead
  • otherwise
  • rather

Similar to So

  •  
  • accordingly
  • consequently
  • hence
  • meanwhile
  • then
  • therefore
  • thus

 


 

List of Conjunctions

English Conjunctions & Cohesive Devices

Conjunctions are also sometimes called - or put in the category of - Linking Words. Please feel free todownload them via this link to the category page:
Linking Words & Connecting Words as a PDF.

It contains all the conjunctions listed on this site. The image to the left gives you an impression how it looks like.

 

 


 

Further helpful readings about vocabulary (examples included): Smart Rephrased Proverbs   ¦   Rare & Funny Tongue Twisters   ¦   Learn Chinese in 5 minutes

 


 

Writing Tip: In order to save precious characters and typing time, there is a rich language of text abbreviations used in Text Messaging, Twitter, SMS and Chat. 
It is quite a long list that probably does not include every Text Abbreviation & Internet Acronym ever invented — but the most popular. Even if you restrain yourself from memorizing and using them all, you might find it handy to know a place where you can look up and thus decipher what someone is writing.

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